Methylene Blue 1%
Inventory Status:
Availability: Available
$32.00
Strict Regulatory Notice: This agent is synthesized strictly for laboratory-based research and analytical purposes. It is not intended for human or veterinary use, nor for medical, household, or cosmetic applications. Please review our Terms of Service thoroughly before finalizing your acquisition.
Composition: Each mL of sterile aqueous solution contains:
Active Ingredients: METHYLENE BLUE 1% 10mg/mL
Inactive Ingredients: Deionized Water USP
Availability: Available
Guaranteed Safe Checkout:
Key Specifications
| Parameter | Specification / Details |
|---|---|
| Primary Compound Name | Methylene Blue (Methylthioninium Chloride) |
| CAS Registry Number | 61-73-4 (Anhydrous), 7220-79-3 (Trihydrate) |
| IUPAC Designation | [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride |
| Synonyms / Trade Names | Swiss Blue, Urolene Blue, Basic Blue 9 |
| PubChem CID | 6099 |
| Molecular Formula | C₁₆H₁₈ClN₃S |
| Molar Mass / Weight | 319.85 g/mol |
| Physical Appearance | Dark blue aqueous solution (Raw powder is dark green/bronze crystalline) |
| Solubility Profile | Readily soluble in water and ethanol. |
| Tested Purity | Medical / USP Grade (Heavy-metal free is critical) |
| Active Ingredient (Total) | 300mg per 30mL vial |
| Dry / Powder Formulations | N/A (Aqueous solution formulation) |
| Liquid / Solution Concentrations | 1% (10mg/mL) typically suspended in highly purified/sterile water |
| Alternative Formulations | 0.5% drops, 2% solutions, oral troches/capsules |
| Storage Guidelines | Store at 20°C to 25°C (68°F to 77°F). Keep tightly closed and protect strictly from direct light. |
| Estimated Shelf Life | 24 to 36 months from manufacture date. |
| Safety Documentation | Standard SDS available for Methylene Blue Solutions |
| Terms of Application | For laboratory research purposes only. Investigated for its role as an electron redox carrier in the mitochondrial electron transport chain and as an inhibitor of nitric oxide synthase and guanylate cyclase. |
Foundational Scientific Overview
Methylene Blue 1% is a prominent subject within the field of mitochondrial bioenergetics and histological staining. Originally synthesized as a phenothiazine derivative to investigate electron transport modulation, it has become a staple for researchers examining cellular respiration, nitric oxide synthase inhibition, and tau protein aggregation.
At 4 Amino Labs, we provide this compound in a highly purified liquid format to ensure experimental reproducibility. By maintaining rigorous measurement standards, we provide the consistency required for longitudinal data integrity. This compound is frequently cited in peer-reviewed literature due to its well-documented role as an alternative electron carrier bypassing complex I-III blockades in the mitochondrial electron transport chain.
Analytical Composition & Purity Standards
- Chemical Formula: C16H18ClN3S
- Physical State: Deep Blue Aqueous Solution
Our Methylene Blue 1% undergoes a multifaceted verification process. Each batch is subjected to independent High-Performance Liquid Chromatography (HPLC) to ensure a purity threshold of ≥ 99%, strictly devoid of the toxic heavy metal impurities (such as arsenic, aluminum, and cadmium) frequently found in lower-grade industrial or chemical variants.
Investigational Pathways
Researchers utilizing Methylene Blue 1% typically focus on the following biochemical mechanisms:
- Mitochondrial Respiration: Evaluating the compound’s influence on oxygen consumption and ATP production during induced hypoxic states.
- Guanylate Cyclase Inhibition: Observing the modulation of vasodilation pathways and cGMP levels in endothelial models.
- Methemoglobin Reduction: Mapping the flux of electron transfer to convert oxidized methemoglobin back to functional hemoglobin in erythrocyte studies.
- Neurodegenerative Modeling: Investigating the agent’s role in preventing the oligomerization of tau proteins in Alzheimer’s-like cellular assays.
Laboratory Handling & Stability Protocol
To maintain the molecular integrity of Methylene Blue 1%, the following storage parameters are recommended:
- Thermal Regulation: Store in a controlled, cool, and desiccated environment.
- Photostability: Highly photosensitive. Protect from direct UV exposure; storage in an opaque container or dark cabinet is heavily advised to prevent photodegradation.
- Contamination Control: Use sterilized instruments and work within a laminar-flow hood, when possible, to maintain sample sterility. Handle with care, as it is a potent stain.
Compliance & Safety Disclaimer
This substance is provided exclusively for in-vitro laboratory research and pre-clinical developmental testing. 4 Amino Lab does not condone or authorize the use of this product in any clinical setting or for personal consumption. Misuse of laboratory chemicals carries significant health risks. By purchasing, the researcher acknowledges the inherent risks and confirms they possess the necessary equipment and expertise to handle these materials safely.
