B12 (Methylcobalamin) 1mg/ml 30mL
Inventory Status:
Availability: Available
$55.50
Strict Regulatory Notice: This agent is synthesized strictly for laboratory-based research and analytical purposes. It is not intended for human or veterinary use, nor for medical, household, or cosmetic applications. Please review our Terms of Service thoroughly before finalizing your acquisition.
Composition: Each mL of sterile aqueous solution contains:
Active Ingredients:
Methylcobalamin (B12)………………1000 mcg
Inactive Ingredients: Benzyl Alcohol 1.5% v/v as preservative, Ammonium Sulfate 0.1%.
Availability: Available
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Key Specifications
| Parameter | Specification / Details |
|---|---|
| Primary Compound Name | Methylcobalamin Injection, USP (Vitamin B12) |
| CAS Registry Number | 13422-55-4 (Methylcobalamin) |
| IUPAC Designation | Coα-[α-(5,6-dimethylbenz-1H-imidazolyl)]-Coβ-cyanocobamide |
| Synonyms / Trade Names | Vitamin B12, Cobamin, Crystamine |
| PubChem CID | 5311498 |
| Molecular Formula | C₆₃H₈₈CoN₁₄O₁₄P |
| Molar Mass / Weight | 1355.38 g/mol |
| Physical Appearance | Sterile, clear, dark red aqueous solution |
| Solubility Profile | Freely soluble in water and alcohol |
| Tested Purity | Medical / USP Grade |
| Active Ingredient (Total) | 30mg (30,000 mcg) Cyanocobalamin per 30mL vial |
| Dry / Powder Formulations | N/A (Aqueous injection formulation) |
| Liquid / Solution Concentrations | 1mg/mL (1000 mcg/mL) Cyanocobalamin. Typically includes a preservative (e.g., 1.5% Benzyl Alcohol) |
| Alternative Formulations | Methylcobalamin, Hydroxocobalamin (injections); oral/sublingual tablets |
| Storage Guidelines | Store at 20°C to 25°C (68°F to 77°F). Protect strictly from light. |
| Estimated Shelf Life | Multidose vials must be discarded 28 days after initial puncture. |
| Safety Documentation | Standard SDS available for Cyanocobalamin Injection |
| Terms of Application | Treatment of Vitamin B12 deficiencies (e.g., pernicious anemia). Administered via Intramuscular (IM) or deep Subcutaneous (SubQ) injection. |
Foundational Scientific Overview
B12 Injection (Cyanocobalamin/Methylcobalamin) is a prominent subject within the field of hematological models and cellular metabolism. As a cobalt-containing coordination compound, it has become a staple for researchers examining DNA synthesis, fatty acid metabolism, and myelin sheath formation in neurological in-vitro assays.
At 4 Amino Labs, we provide this compound in a highly stable aqueous format to ensure experimental reproducibility. By maintaining rigorous measurement standards, we provide the consistency required for longitudinal data integrity. This compound is frequently cited in peer-reviewed literature for its role as an essential coenzyme for methionine synthase and L-methylmalonyl-CoA mutase.
Analytical Composition & Purity Standards
Chemical Formula: C63H88CoN14O14P
Physical State: Red Aqueous Solution
Our B12 Injection undergoes a multifaceted verification process. Each batch is subjected to independent High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) to ensure a purity threshold of ≥ 99%. This analytical rigor ensures the absence of heavy metals or cross-contaminants, providing a stable baseline for your in-vitro or pre-clinical models.
Investigational Pathways
Investigational Pathways
Researchers utilizing the B12 Injection typically focus on the following biochemical mechanisms:
- Methyl Group Transfer: Evaluating the compound’s role in converting homocysteine to methionine within the folate cycle.
- Mitochondrial Bioenergetics: Observing the mutase-driven conversion of methylmalonyl-CoA to succinyl-CoA, a crucial step in the citric acid cycle.
- Erythropoiesis Modeling: Mapping the flux of erythroblast maturation and DNA replication rates in marrow-derived cellular cultures.
- Neuroprotection: Investigating the agent’s capacity to support lipid synthesis required for myelin repair in demyelination assays.
Laboratory Handling & Stability Protocol
To maintain the molecular integrity of the B12 Injection, the following storage parameters are recommended:
- Thermal Regulation: Store in a controlled room-temperature environment (20°C to 25°C). Do not freeze.
- Photostability: Highly photosensitive; prolonged exposure to light will cleave the cobalt bonds. Storage in an opaque container or dark cabinet is strictly advised.
- Hygroscopic Prevention: Ensure the seal is hermetic after every withdrawal to prevent environmental contamination.
- Contamination Control: Use sterilized instruments and work within a laminar-flow hood, when possible, to maintain sample sterility.
Compliance & Safety Disclaimer
This substance is provided exclusively for in-vitro laboratory research and pre-clinical developmental testing. 4 Amino Lab does not condone or authorize the use of this product in any clinical setting or for personal consumption. Misuse of laboratory chemicals carries significant health risks. By purchasing, the researcher acknowledges the inherent risks and confirms they possess the necessary equipment and expertise to handle these materials safely.
