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Strict Regulatory Notice: This agent is synthesized strictly for laboratory-based research and analytical purposes. It is not intended for human or veterinary use, nor for medical, household, or cosmetic applications. Please review our Terms of Service thoroughly before finalizing your acquisition.
Composition: Each mL of sterile aqueous solution contains:
Active Ingredients: METHYLENE BLUE 1% 10mg/mL
Inactive Ingredients: Deionized Water USP
Available
| Parameter | Specification / Details |
|---|---|
| Primary Compound Name | Methylene Blue (Methylthioninium Chloride) |
| CAS Registry Number | 61-73-4 (Anhydrous), 7220-79-3 (Trihydrate) |
| IUPAC Designation | [7-(dimethylamino)phenothiazin-3-ylidene]-dimethylazanium;chloride |
| Synonyms / Trade Names | Swiss Blue, Urolene Blue, Basic Blue 9 |
| PubChem CID | 6099 |
| Molecular Formula | C₁₆H₁₈ClN₃S |
| Molar Mass / Weight | 319.85 g/mol |
| Physical Appearance | Dark blue aqueous solution (Raw powder is dark green/bronze crystalline) |
| Solubility Profile | Readily soluble in water and ethanol. |
| Tested Purity | Medical / USP Grade (Heavy-metal free is critical) |
| Active Ingredient (Total) | 300mg per 30mL vial |
| Dry / Powder Formulations | N/A (Aqueous solution formulation) |
| Liquid / Solution Concentrations | 1% (10mg/mL) typically suspended in highly purified/sterile water |
| Alternative Formulations | 0.5% drops, 2% solutions, oral troches/capsules |
| Storage Guidelines | Store at 20°C to 25°C (68°F to 77°F). Keep tightly closed and protect strictly from direct light. |
| Estimated Shelf Life | 24 to 36 months from manufacture date. |
| Safety Documentation | Standard SDS available for Methylene Blue Solutions |
| Terms of Application | For laboratory research purposes only. Investigated for its role as an electron redox carrier in the mitochondrial electron transport chain and as an inhibitor of nitric oxide synthase and guanylate cyclase. |
Methylene Blue 1% is a prominent subject within the field of mitochondrial bioenergetics and histological staining. Originally synthesized as a phenothiazine derivative to investigate electron transport modulation, it has become a staple for researchers examining cellular respiration, nitric oxide synthase inhibition, and tau protein aggregation.
At 4 Amino Labs, we provide this compound in a highly purified liquid format to ensure experimental reproducibility. By maintaining rigorous measurement standards, we provide the consistency required for longitudinal data integrity. This compound is frequently cited in peer-reviewed literature due to its well-documented role as an alternative electron carrier bypassing complex I-III blockades in the mitochondrial electron transport chain.
Our Methylene Blue 1% undergoes a multifaceted verification process. Each batch is subjected to independent High-Performance Liquid Chromatography (HPLC) to ensure a purity threshold of ≥ 99%, strictly devoid of the toxic heavy metal impurities (such as arsenic, aluminum, and cadmium) frequently found in lower-grade industrial or chemical variants.
Researchers utilizing Methylene Blue 1% typically focus on the following biochemical mechanisms:
To maintain the molecular integrity of Methylene Blue 1%, the following storage parameters are recommended:
This substance is provided exclusively for in-vitro laboratory research and pre-clinical developmental testing. 4 Amino Lab does not condone or authorize the use of this product in any clinical setting or for personal consumption. Misuse of laboratory chemicals carries significant health risks. By purchasing, the researcher acknowledges the inherent risks and confirms they possess the necessary equipment and expertise to handle these materials safely.
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